[Assay of adenine nucleotide level in platelet storage pool for evaluation of the course of hemoblastosis]. / Issledovanie urovnia adeninnukleotidov pula khraneniia trombotsitov dlia otsenki techeniia gemoblastozov.

The content of adenine nucleotides (ATP, ADP) was studied in dense granules of platelets in hemoblastosis to estimate the character of the pathological process course. Varying biochemical defects were observed at the levels of ATP and ADP depending on the severity of the pathological process in chronic myeloid leukemia, acute lymphoblastic leukemia, acute myeloblastic and acute myelo-monoblastic leukemias. Adenine nucleotide values can be used for the diagnosis of varying stages of the above diseases, for the evaluation of anomalous platelets and characterization of the adequacy of the bone marrow hemopoiesis.

[Importance of studying the level of platelet adenine nucleotide levels in various phases of chronic myeloleukoses]. / Znachenie issledovaniia urovniia adeninnukleotidov trombotsitov v raznykh fazakh zabolevaniia khronicheskim mieloleikozom.

Content of adenine nucleotides was studied in various compartments of blood platelets obtained from donors and patients with chronic myeloleukosis at various steps of the disease. Dissimilar biochemical defect was found in content of ATP and ADP, which varied in thrombocyte storage granules depending on severity of the disease. The data on adenine nucleotides content may be used for diagnosis of various steps of chronic myeloleukosis, for detection of thrombocytes anomaly and for evaluation of the state of medullary hemopoiesis.

[Physico-chemical and catalytic properties of hypoxanthine guanine phosphoribosyltransferase in thrombocytes from blood donors and patients with hemophilia A]. / Fiziko-khimicheskie i kataliticheskie svoistva gipoksantinguaninfosforiboziltransferazy trombotsitov donorov i bol'nykh gemofiliei A.

Studies of some physico-chemical and catalytic properties of hypoxanthine guanine phosphoribosyl transferase HGPRT) in thrombocytes of donors and patients with hemophilia A showed that kinetic parameters (Km, Vmax) of the enzyme were similar both under normal and pathological conditions. However, some differences were detected in thermolability and inhibition of HGPRT by purine metabolites: IMP, GMP and inosine. These data suggest that molecular impairment may occur in HGPRT under conditions of hemophilia A.

[The role of adenine nucleotides in the functional activity of platelets in hemophilia A]. / Znachenie issledovaniia adeninnukleotidov v funktsional'noi aktivnosti trombotsitov pri gemofilii A.

Content of adenine nucleotides was studied in various pools of thrombocytes from donors and from patients with hemophilia A. Biochemical deficiency of adenine nucleotides appears to occur in the pool of thrombocytes obtained from patients with hemophilia A; estimation of ADP and ATP in the storage granules as well as of the nucleotides specific radioactivity may serve as a criterion of thrombocyte disfunction.

[Adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase of blood platelets in hereditary coagulopathies]. / Adeninfosforiboziltransferaza i gipoksantin-guaninfosforiboziltransferaza trombotsitov krovi pri nasledstvennykh koagulopatiiakh.

Activities of adenine phosphoribosyltransferase (EC 2.4.2.7 APRT) and hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8 HGPRT) were studied in thrombocytes of healthy donors, patients with hemophilia A and B and of women--heterozygote carriers of the pathologic gene. The data obtained suggest that HGPRT test may be used as a genetic marker of hemophilia as well as to detect the heterozygote carriers; estimation of APRT activity is suitable test for differentiation of hemophilia forms.